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anti human ccl22 neutralizing antibody  (R&D Systems)


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    R&D Systems anti human ccl22 neutralizing antibody
    Anti Human Ccl22 Neutralizing Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mdc/Human+CCL22%2FMDC+Antibody/pm42815071-107-31-35
    Average 93 stars, based on 12 article reviews
    anti human ccl22 neutralizing antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Photinia glabra -derived exosome-like nanovesicles mitigate skin inflammaging via dual regulation of inflammatory signaling and calcium homeostasis.
    Article Snippet: Aims: Chronic low-grade inflammation accelerates skin aging, termed inflammaging.. This study investigates whether Photinia glabra-derived exosome-like nanovesicles (PgELNs) can alleviate inflammaging by modulating inflammatory signaling and calcium homeostasis.. Materials and methods: PgELNs were isolated using tangential flow filtration and characterized by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM).

    Article Title: Photinia glabra -derived exosome-like nanovesicles mitigate skin inflammaging via dual regulation of inflammatory signaling and calcium homeostasis
    Article Snippet: .. The expression levels of RANTES (Cat# DY278) and MDC (Cat# DY336) were measured using commercial ELISA kits (R&D Systems, Minneapolis, MN, USA) in accordance with the manufacturer’s instructions. .. Fluorescence intensity was detected using an ELISA microplate reader (Synergy HTX Multi-Mode Reader, BioTek, Winooski, VT, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Photinia glabra -derived exosome-like nanovesicles mitigate skin inflammaging via dual regulation of inflammatory signaling and calcium homeostasis.
    Article Snippet: Aims: Chronic low-grade inflammation accelerates skin aging, termed inflammaging.. This study investigates whether Photinia glabra-derived exosome-like nanovesicles (PgELNs) can alleviate inflammaging by modulating inflammatory signaling and calcium homeostasis.. Materials and methods: PgELNs were isolated using tangential flow filtration and characterized by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM).

    Article Title: Dendritic cell proliferation by primary cilium in atopic dermatitis
    Article Snippet: Cytokine/chemokine multiplex array was performed using the Inflammation 20-Plex Human ProcartaPlex Panel (EPX200-12185-901, Invitrogen) and analyzed with a Bio-Plex 200 (BioRad) following manufacturer protocols. .. ELISA kits for CCL17 (DY364), MCP1 (DCP00), MDC (DMD00), IL-4 (D4050), and GM-CSF (DY215) were purchased from R&D Systems. .. PDGF-A ELISA kits (EHPDGF) were purchased from Thermo Fisher Scientific.

    Article Title: Fermented Platycodon grandiflorum extract alleviates TNF-α/IFN-γ-induced inflammatory response in HaCaT cells and modulates immune balance on 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis in NC/Nga mice
    Article Snippet: .. Then, the medium was transferred into a plate and cytokines and chemokines, including IL-6 (cat# D6050), IL1β (cat# DLB50), MDC (cat# DMD00), and TARC (cat# DDN00) were measured using an enzyme-linked immunosorbent assay (ELISA) kit (R&D system) according to the manufacturer’s protocols. ..

    Article Title: Photinia glabra -derived exosome-like nanovesicles mitigate skin inflammaging via dual regulation of inflammatory signaling and calcium homeostasis
    Article Snippet: .. The expression levels of RANTES (Cat# DY278) and MDC (Cat# DY336) were measured using commercial ELISA kits (R&D Systems, Minneapolis, MN, USA) in accordance with the manufacturer’s instructions. .. Fluorescence intensity was detected using an ELISA microplate reader (Synergy HTX Multi-Mode Reader, BioTek, Winooski, VT, USA).

    Article Title: Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation
    Article Snippet: .. TNF-α (Cat#: MTA00B), IL-1β (Cat#: MLB00C), IL-6 (Cat#: M6000B), IL-18 (Cat#: DY122-05), MCP-1 (Cat#: SMJE00B), CCL1 (Cat#: DY845), IL-17 (Cat#: SM1700), IL-2 (Cat#: SM2000), IL-10 (Cat#: SM1000B), IL-5 (Cat#: SM5000), MIP-2 (Cat#: SMM200), MIP-3β (Cat#: DY440), MDC (Cat#: MCC220), CX3CL1 (Cat#: MCX310), CXCL10 (Cat#: DY466-05), and CXCL12 (Cat#: DY460) ELISA kits were purchased from R&D Systems (Shanghai, China) and used according to the product specifications. .. An MCP-3 (Cat#: BMS6006INST) ELISA kit was acquired from Invitrogen.

    Article Title: Anti-inflammatory effects of ethanol extract from the leaves and shoots of Cedrela odorata L. in cytokine-stimulated keratinocytes
    Article Snippet: .. The inhibitory effect of COEE on the secretion of interleukin (IL)-6, IL-8, TARC and MDC into the supernatants was evaluated using the following ELISA kits: IL-6 (cat. no. 555220; BD Biosciences, Santa Clara, CA, USA), IL-8 (cat. no. DY208), TARC (cat. no. DY364) and MDC (cat. no. DY336; all R&D Systems, Inc., Minneapolis, MN, USA). ..

    other:

    Article Title: Dendritic cell proliferation by primary cilium in atopic dermatitis
    Article Snippet: After 5 days of differentiation, cells were electroporated with 20 nM siRNA targeting human IFT88 (Life Technologies), or siRNA Control (AM4611, Invitrogen) with the Neon Transfection System (Thermo Fisher Scientific).



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    Regulatory effect of DML <t>on</t> <t>autophagy</t> in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by <t>MDC</t> staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.
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    Regulatory effect of DML <t>on</t> <t>autophagy</t> in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by <t>MDC</t> staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.
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    R&D Systems anti ccl22 mdc antibody
    Regulatory effect of DML <t>on</t> <t>autophagy</t> in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by <t>MDC</t> staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.
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    Image Search Results


    Regulatory effect of DML on autophagy in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by MDC staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.

    Journal: ACS Omega

    Article Title: Des-O-Methyllasiodiplodin Isolated from Xanthostemon chrysanthus Ameliorates Inflammation by Regulating Autophagy

    doi: 10.1021/acsomega.5c12134

    Figure Lengend Snippet: Regulatory effect of DML on autophagy in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by MDC staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.

    Article Snippet: Reactive Oxygen Species Assay Kit, DAF-FM DA probe, Autophagy Staining Assay Kit with MDC, RIPA lysate buffer, BCA protein determination kit, and All-purpose Powerful Antigen Retrieval Solution were from Beyotime Institute of Biotechnology (Shanghai, China).

    Techniques: Staining, Confocal, Microscopy, Fluorescence, Expressing, Analysis, Control